Authors: Côme PASQUALIN - François GANNIER (gannier at univ-tours dot fr) University of Tours (France) See also: Others productions for ImageJ from the authors Utilisation: Description / Installation / Utilisation of the plugin Description: Spiky can help to analyse echocardiography images by the measurement of the change in surface cavities of the heart.
Example
Peak analysis of left ventricular (LV) area variation computed from echocardiography.
Procedure Results table of peak analysis.
Requirements: File must be a stack and must be calibrated in each dimension (Use Ctrl+Shift+P) .
This analysis starts from an unique point chosen when you perform a right click to start the contextual menu.
Before using tool: You need to select "wand tool ", left click on the area, then double click the wand tool and set the "wand tolerance ": change progressively the tolerance to see the selected zone (keep a correct margin). Valid the option box. This value of tolerance will be used to detect the area of interest.
Using tool: Place the cursor inside the correct zone and right click, choose "Plot Result from Wand" from contextual menu to get the plot.
After using tool: When the plot is correct, use "Peak analysis".
Tips: If the plot isn't correct:
- try to right click on a different point.
- try to change (generally lower) the tolerance.
- if the image has a poor contrast, the wand tool cannot detect correctly the edge of the area, so try to slightly rise the contrast of black values. For that, use "Brightness and Contrast " but only for minimal values. Do not forget to apply on all images in the stack.
- if only some points are bad, try the wand tool on another point inside of the corresponding image.
- if only few points are bad, use the list button, clear each bad values, then use the "plot result" button.
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